What is BPG column?

BPG columns are glass chromatography columns designed for industrial applications, which demand high standards of hygiene. The columns are constructed from component materials of the highest quality and withstand the harsh conditions used for cleaning in place of packed downstream purification resins.

Why do we store chromatography columns in 20% ethanol?

Bacteria thrive in the neutral environment of buffers. Prevent bacterial growth by running water through the column. Then, place columns in bacteriostatic solutions, such as those containing 20% ethanol, and in the refrigerator at 4 oC to 8 oC, he says.

What is a chromatography column made of?

Materials. Liquid chromatography: Traditional chromatography columns were made of glass. Modern columns are mostly made of borosilicate glass, acrylic glass or stainless steel. To prevent the stationary phase from leaking out of the column interior a polymer, stainless steel or ceramic net is usually applied.

How do you clean the size of the exclusion column?

If the column is to be stored for more than 2 d after use, wash it with 2 CV of water and then equilibrate with at least 2 CV of 20% ethanol (for HiLoad Superdex 30 pg and Superdex 75 pg, use 200 mM sodium acetate in 20% ethanol). Note: Use a lower flow rate for viscous 20% ethanol.

How do you store columns in chromatography?

Keep it tightly capped when not in use. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth. Use the column in the direction indicated on the label. An unusually high operating pressure may indicate a plugged inlet frit and may be cleared by reversing flow through the column for 10-20 mL.

What are the 4 types of column chromatography?

Column chromatography is therefore classified according to the type of fluid flow system used:

• Gravity chromatography.
• Low-pressure chromatography.
• Medium-pressure chromatography (including fast protein liquid chromatography)
• High-pressure/high-performance liquid chromatography (HPLC)

What size column do I need chromatography?

What size column do I need?

Column Size (mL)	Fraction Size (mL)	Sample size
Separation
2000	100	20-30
4000	150-200	50
8000	250-400	100

How do you clean a Superdex 200 column?

Cleaning is performed at low flow rates using 1 CV of sodium hydroxide (500 mM sodium hydroxide for Superdex and Superose columns and 200 mM sodium hydroxide for Sephacryl columns). Note that the column should never be stored in sodium hydroxide.

How do you clean a column filter?

remove the frit of the guard column & sonicate whole guard column assembly in 5% H2SO4 for 15-20min then sonicate in 100% water to remove acid and then with 100% methanol. Pressure will definitely get reduce.

How should columns be stored?

Normal phase columns should be stored in Hexane. Reversed phase stationary phases should be kept in a mixture of water / organic modifier (Acetonitrile/Methanol), free from any buffers or additives that might precipitate during storage.

How do I preserve a column in HPLC?

HPLC Column Care & Storage

1. Routinely monitor the column’s performance.
2. Switch only between miscible mobile phases.
3. Avoid precipitating salts in the column.
4. Use filtered and degassed mobile phases.
5. Do not allow the column to dry out.
6. For prolonged storage, use a mobile phase that inhibits bacterial and mold growth.

Which column is used in HPLC?

HPLC columns are mostly four main types Normal phase, Reverse Phase, and Ion Exchange columns. In the pharmaceutical industry mostly reverse columns are used for analysis..

What is the flow rate of a BPG 300 column?

Figure. BPG 300, Accessories for Running the column, parts identification. For flow rates 3 l/h-175 l/h use Low Flow Kit 28-9301-82. For flow rates 7.5 l/h-510 l/h use High Flow Kit 28-9301-83.

What are the specifications of BPG column?

Specifications: Scalable from 100 mm to 450 mm Pressure rating up to 8 bar Glass column tube allows efficient and reproducible packing Hygienic design and operation. Microbial attachment and growth is prevented t… Used BPG Chromatography Column designed for industrial applications which demand high standards of hygiene.

What size gasket do I need for a BPG 300 column?

For High Flow Kit use 4-port, 2-way Valve i.d. 10 mm, 25 mm TC 18-1012-56. For Low Flow Kit use gasket with i.d. 6 mm 18-0019-27 or i.d. 8 mm 28-9509-48. For High Flow Kit use gasket with i.d. 10 mm 18-1035-79. Figure 2. BPG 300 Column parts identification. Item numbers are not necessarily in sequential order.

Which bpg valves are suitable for my bpg column?

The 2-way or 4-way valves with 6 mm internal diameter are suitable for BPG 100, 140, and 200 columns. The 10 mm internal diameter is suitable for BPG 300 and 450 columns.