What is cell line identification?
What is cell line identification?
Cell lines can be tested and identified using multiallelic variable number of tandem repeats (VNTR). HLA typing and DNA fingerprinting using short tandem repeat (STR) and a variable number of tandem repeats for intra-species cross-contamination have been used for cell line identification.
How do you authenticate a cell phone line?
The main test for authenticating cell lines is short tandem repeat (STR) profiling, the analysis of microsatellite regions of DNA that have variable numbers of repeats and are located throughout the genome.
What is an STR profile?
STR profiling is an analytical DNA technique which PCR-amplifies variable microsatellite regions from a genomic DNA template, separates the PCR amplicons on a genetic analyzer, and uses software to analyze the resulting data and compare the data from one specimen to databases housing previously generated STR sets.
Which benchmark cell line verification is recommended by ATCC?
Identity verification with STR analysis (DNA fingerprinting) for human cell lines.
What is STR profile of a cell line?
STR profiling is a standard procedure for cell line authentication (ANSI/ATCC ASN-002). Cell line authentication provides researchers with confidence that their cell lines are correctly identified, and not cross contaminated with other cells.
How does STR analysis work?
As the name implies, short tandem repeat (STR) analysis is a method of determining an individual’s DNA profile by counting the number of times a small DNA sequence (short tandem repeat unit) is repeated at a specific chromosomal location.
Why do you feel necessary to authenticate a cell line?
Contamination and misidentification of cell lines Research results based on contaminated or misidentified cell lines most likely lead to false interpretations, correlations and conclusions (da Silva, 2018; Routray, et al., 2016).
Why is cell line characterization important?
Why is cell line characterization important? Cell line characterization is critical to the control of biological product manufacturing. The objective of the testing is to confirm the identity, purity, and suitability of the cell substrate for manufacturing use.
Where are STRs found?
Most STRs are found in the noncoding regions, while only about 8% locate in the coding regions (3). Moreover, their densities vary slightly among chromosomes. In humans, chromosome 19 has the highest density of STRs (4). On average, one STR occurs per 2,000 bp in the human genome (5).
What are STRs in DNA?
The most common type of DNA profiling today for criminal cases and other types of forensic uses is called “STR” (short tandem repeat) analysis. Using DNA to distinguish between two individuals is a tricky matter, because close to 99.9 percent of our DNA is the same as everybody else’s DNA.
Are ATCC cell lines authenticated?
Unlike other providers, we authenticate cell lines by comparing the STR profile of sample cell lines with the ATCC Human Cell STR Database. If your cell lines are not from ATCC, we can still conduct a comprehensive interpretation of results.