What is pen strep used for?
What is pen strep used for?
Pen-Strep (also known as penicillin-streptomycin) is a mixture of penicillin G and streptomycin that is widely used in mammalian cell culture media to prevent bacterial contamination.
How do you administer a strep Pen?
Administer by deep intramuscular injection. Recommended dosage rate is 8 mg/kg bodyweight procaine penicillin with 10 mg/kg bodyweight dihydrostreptomycin sulphate equivalent to 1 ml per 25 kg bodyweight. Treatment should be given once daily for up to 3 consecutive days.
Is pen strep an antibiotic?
Pen/strep is the dream team of antibiotics for cell culture purposes. Where penicillin interferes with the turnover of bacterial walls, streptomycin binds 30S subunits of bacterial ribosomes—the combination of both results in the inhibition of protein synthesis and ultimately the death of susceptible bacteria.
Can you give dogs Pen and strep?
Dosage and Administration Cattle, sheep, goats, horses, camelids and swine: 1 mL/25 kg of b.w. by intramuscular route; dogs and cats: 1 mL/10 kg of b.w. Apply through intramuscular route (subcutaneous route could be used in dogs).
Is Pen and Strep long acting?
Meat: 31 days. Cattle, Milk: 60 hours. Milk for human consumption must not be taken during treatment. Meat: 23 days.
How long does Pen-Strep last in media?
12 months
Finally, I know media (DMEM) by itself is generally stable for 12 months from manufacture when stored refrigerated, and sera is generally stable for 5 years when stored at -20°C or colder.
How much penicillin do I give a cow?
DOSAGE: The dosage for cattle, sheep, swine, and horses is 3000 units per pound of body weight, or 1.0 mL for each 100 pounds of bodyweight, once daily.
Is Pen and strep long acting?
How much penicillin can you give a dog injectable?
The daily dose of penicillin is 3,000 units per pound of body weight (1 mL per 100 lbs body weight). Continue daily treatment until recovery is apparent and for at least one day after symptoms disappear, usually in two to three days.
Does penicillin make dogs sleepy?
In many pets, antibiotics can cause lethargy, nausea and/or a loss of appetite, which may make your pet less interested in eating their food. But it is crucial that your pet continues to eat, because their body needs the energy to continue fighting infection and repairing damaged cells.
What is the withdrawal on Pen and Strep?
Withdrawal Period Meat: 31 days. Cattle, Milk: 60 hours. Milk for human consumption must not be taken during treatment.
What is the withdrawal period for pen strep?
Animals must not be slaughtered for human consumption during treatment. Cattle intended for human consumption should not be slaughtered until 23 days after the last treatment. Pigs intended for human consumption should not be slaughtered until 18 days after the last treatment.