What is the role of p62 oligomerization in autophagy?
What is the role of p62 oligomerization in autophagy?
Oligomerization of p62 allows for selection of ubiquitinated cargo and isolation membrane during selective autophagy.
What is ATG in autophagy?
This nomenclature has been adopted in most other eukaryotic systems, further simplifying the naming of these genes and proteins. As noted in the nomenclature paper, “ATG” and “Atg” stand for “autophagy-related” gene or protein, respectively. That is, “ATG” means “autophagy-related,” and that is it.
What is the autophagic pathway?
Briefly, the autophagy pathway entails the development of a phagophore that envelopes cytoplasmic components and forms a double-membrane autophagosome that subsequently fuses with a lysosome for the digestion of its contents.
What is cell autophagy?
Autophagy is a fundamental cell survival mechanism that allows cells to adapt to metabolic stress through the degradation and recycling of intracellular components to generate macromolecular precursors and produce energy.
What is p62 autophagy?
p62, a classical receptor of autophagy, is a multifunctional protein located throughout the cell and involved in many signal transduction pathways, including the Keap1–Nrf2 pathway. It is involved in the proteasomal degradation of ubiquitinated proteins.
Is p62 a marker for autophagy?
Since p62 accumulates when autophagy is inhibited, and decreased levels can be observed when autophagy is induced, p62 may be used as a marker to study autophagic flux.
What is p62 in autophagy?
p62, a classical receptor of autophagy, is a multifunctional protein located throughout the cell and involved in many signal transduction pathways, including the Keap1-Nrf2 pathway. It is involved in the proteasomal degradation of ubiquitinated proteins.
What is LC3 autophagy?
Microtubule-associated protein 1A/1B-light chain 3 (LC3) is a soluble protein with a molecular mass of approximately 17 kDa that is distributed ubiquitously in mammalian tissues and cultured cells. During autophagy, autophagosomes engulf cytoplasmic components, including cytosolic proteins and organelles.
What is the autophagic pathway and why is it necessary?
Macroautophagy/autophagy is an essential, conserved self-eating process that cells perform to allow degradation of intracellular components, including soluble proteins, aggregated proteins, organelles, macromolecular complexes, and foreign bodies.
What are the different types of autophagy?
The evolutionarily conserved process for the elimination of damaged and redundant components in cells is known as autophagy. Currently three forms of autophagy are described: macroautophagy, microautophagy, and chaperone-mediated autophagy.
Is autophagy programmed cell death?
Autophagic cell death (also known as Type II programmed cell death to distinguish it from apoptosis or Type I programmed cell death) (20-22) has been described as a distinct form of cell death that differs from other death mechanisms such as apoptosis and necrosis.
Is autophagy a type of cell death?
The Nomenclature Committee of Cell Death defines autophagy-dependent cell death as ‘a form of regulated cell death that mechanistically depends on the autophagic machinery (or components thereof)’ [10].