What is the difference between phospholipase A1 and A2?

What is the difference between phospholipase A1 and A2?

Phospholipase A1 – cleaves the sn-1 acyl chain (where sn refers to stereospecific numbering). Phospholipase A2 – cleaves the sn-2 acyl chain, releasing arachidonic acid.

What is the function of phospholipase A1?

In particular, phospholipase A1 (PLA1) specifically catalyzes the cleavage at the SN-1 position of phospholipids, forming a fatty acid and a lysophospholipid.

What is the role of the enzyme phospholipase A2?

Phospholipase A2 (PLA2) plays crucial roles in diverse cellular responses, including phospholipid digestion and metabolism, host defense and signal transduction.

What does phospholipase A1 release?

Phospholipase A1 (PLA1) is an enzyme that hydrolyzes phospholipids and produces 2-acyl-lysophospholipids and fatty acids.

Where does phospholipase A2 cleave?

Phospholipases A2 (PLA2s) EC 3.1. 1.4 are enzymes that cleave fatty acid in position two of phospholipids, hydrolyzing the bond between the second fatty acid “tail” and the glycerol molecule.

What enzyme breaks triglycerides?

Lipoprotein lipase
Lipoprotein lipase breaks down triglycerides carried by two different types of lipoproteins, which bring fat to the bloodstream from different organs.

What does the lipoxygenase pathway produce?

The 5-lipoxygenase protein (5LO) inserts molecular oxygen into free and esterified fatty acids with the aid of 5-lipoxygenase activating protein (FLAP). 5-lipoxygenase acts on arachidonic acid to produce 5-HPETE, which is metabolized into 5-HETE or leukotriene A4, depending on the cellular milieu.

Which protein activates phospholipase A2?

Gβ1γ2 activates phospholipase A2-dependent Golgi membrane tubule formation. Heterotrimeric G proteins transduce the ligand binding of transmembrane G protein coupled receptors into a variety of intracellular signaling pathways.

What does phospholipase A2 cleave?

Phospholipase A2 (PLA2), which cleaves phospholipids producing lysophospholipids and free fatty acids, was originally identified as an intracellular protein involved in cell signaling and in the production of free fatty acids, such as arachidonic acid.

How does phospholipase A2 affect the cell membrane?

Phospholipases A2 (PLA2s) are essential enzymes in cells. They are not only responsible for maintaining the structural organization of cell membranes, but also play a pivotal role in the regulation of cell functions.

What enzymes break down lipids?

Lipase is an enzyme the body uses to break down fats in food so they can be absorbed in the intestines. Lipase is produced in the pancreas, mouth, and stomach.

How are triacylglycerols broken down?

Triglycerides cannot pass through cell membranes freely, and LPLs, special enzymes on the walls of blood vessels, must break triglycerides down into free fatty acids and glycerol; fatty acids can then be taken up by cells via fatty acid transporters.

Phospholipase A1 – cleaves the sn -1 acyl chain (where sn refers to stereospecific numbering ). Phospholipase A2 – cleaves the sn -2 acyl chain, releasing arachidonic acid. Phospholipase B – cleaves both sn -1 and sn -2 acyl chains; this enzyme is also known as a lysophospholipase.

What is the function of phospholipase A2 in bees?

Phospholipase A2 acts on the intact lecithin molecule and hydrolyzes the fatty acid esterified to the second carbon atom. The resulting products are lysolecithin and a fatty acid. Phospholipase A2 is an enzyme present in the venom of bees and viper snakes.

What is the function of phospholipase A 1?

The phospholipase A 1 (PLA 1) family members are esterases that liberate the sn-1 acyl chain from phospholipids, resulting in the formation of a fatty acid and sn -2 acyl lysophospholipid. PLA 1 s are found in mammals, snake and insect venoms and parasites.

Does metal ion and salt affect phospholipase A2 activity?

Metal ion and salt effects on the phospholipase A2, lysophospholipase, and transacylase activities of human cytosolic phospholipase A2. Biochim. Biophys. Acta.1167272–280.